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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
Tslp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal antibodies against tslp  (Santa Cruz Biotechnology)
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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
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rabbit polyclonal (igg) antibody to detect tslp  (Millipore)
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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
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tslp antibody rabbit polyclonal cat# a13134  (ABclonal Biotechnology)
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MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array <t>TSLP</t> <t>and</t> <t>IL-1α</t> analysis (n=3) (** p < 0.01, **** p < 0.0001).
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Image Search Results


MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array TSLP and IL-1α analysis (n=3) (** p < 0.01, **** p < 0.0001).

Journal: Journal of Experimental Pharmacology

Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

doi: 10.2147/JEP.S550568

Figure Lengend Snippet: MβCD/IL-mix-induced AD-like phenotypes and expression of related genes in 3D epidermal models. ( a ) Histological sections of 3D models treated with different concentrations of MβCD/IL-4/IL-13/IL-25. 25: MβCD (7.5 mM), IL-4 (25 ng/mL), IL-13 (25 ng/mL), IL-25 (10 ng/mL); 50: MβCD (7.5 mM), IL-4 (50 ng/mL), IL-13 (50 ng/mL), IL-25 (20 ng/mL); 100: MβCD (7.5 mM), IL-4 (100 ng/mL), IL-13 (100 ng/mL), IL-25 (40 ng/mL). Scale bar 50 µm (n=3). ( b ) qPCR analysis (n=4) (* p < 0.05, ** p < 0.01, **** p < 0.0001, n.s., not significant). ( c ) Cytokine array TSLP and IL-1α analysis (n=3) (** p < 0.01, **** p < 0.0001).

Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

Techniques: Expressing

AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. ( a ) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., not significant). ( b and c ) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Journal of Experimental Pharmacology

Article Title: Artemisia annua Extract Ameliorates Atopic Dermatitis: Evidence from 3D Epidermal Model and Complementary in vitro Assays

doi: 10.2147/JEP.S550568

Figure Lengend Snippet: AAE partially improved the AD-related cytokine expression in AD-like skin equivalent model. ( a ) Cytokine array TSLP, IL-1α, IL-6 and IL-8 analysis (n=3) (** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s., not significant). ( b and c ) Protein expressions of IL-1α and TSLP determined by Western blot (n=4) (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Protein concentration was determined by BCA assay, and cell lysates were resolved by SDS-PAGE on 4–12% gradient Bis-Tris gels (GenScript, M000138), transferred to PVDF membranes, and probed with antibodies against filaggrin (ThermoFisher, PA5-115235), IL-1α and TSLP (Cell Signaling Technology, 34819S and 97630S), p38 MAPK and p-p38 MAPK (Proteintech, 66234-1-Ig and 28796-1-AP), GAPDH (Cell Signaling Technology, 14C10), β-actin (Servicebio, GB15001-100), anti-rabbit IgG and anti-mouse IgG HRP-linked Antibody (Cell SignalingTechnology, 7074P2 and 7076P2).

Techniques: Expressing, Western Blot